LAD1 (NM_005558) Human Mass Spec Standard

CAT#: PH313031

LAD1 MS Standard C13 and N15-labeled recombinant protein (NP_005549)

Product Images

Coomassie blue staining of purified LAD1 protein (Cat# TP313031). The protein was produced from HEK293T cells transfected with LAD1 cDNA clone (Cat# RC213031) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC213031
• Predicted MW: 57 kDa
• Protein Sequence:
  Sequence Link

  >RC213031 representing NM_005558
  Red=Cloning site Green=Tags(s)
  
  MAVSRKDWSALSSLARQRTLEDEEEQERERRRRHRNLSSTTDDEAPRLSQNGDRQASASERLPSVEEAEV
  PKPLPPASKDEDEDIQSILRTRQERRQRRQVVEAAQAPIQERLEAEEGRNSLSPVQATQKPLVSKKELEI
  PPRRRLSREQRGPWALEEESLVGREPEERKKGVPEKSPVLEKSSMPKKTAPEKSLVSDKTSISEKVLASE
  KTSLSEKIAVSEKRNSSEKKSVLEKTSVSEKSLAPGMALGSGRRLVSEKASIFEKALASEKSPTADAKPA
  PKRATASEQPLAQEPPASGGSPATTKEQRGRALPGKNLPSLAEQGASDPPTVASRLPPVTLQVKIPSKEE
  EADMSSPTQRTYSSSLKRSSPRTISFRMKPKKENSETTLTRSASMKLPDNTVKLGEKLERYHTAIRRSES
  VKSRGLPCTELFVAPVGVASKRHLFEKELAGQSRAEPASSRKENLRLSGVVTSRLNLWISRTQESGDQDP
  QEAQKASSATERSQWGQKSDSSLDAEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005549
• RefSeq Size: 2869
• RefSeq ORF: 1551
• Synonyms: LadA
• Locus ID: 3898
• UniProt ID: O00515
• Cytogenetics: 1q32.1
• Summary: 'The protein encoded by this gene may be an anchoring filament that is a component of basement membranes. It may contribute to the stability of the association of the epithelial layers with the underlying mesenchyme. [provided by RefSeq, Jul 2008]'
• Protein Families: Secreted Protein