CTNS (NM_004937) Human Mass Spec Standard

CAT#: PH313151

CTNS MS Standard C13 and N15-labeled recombinant protein (NP_004928)

Product Images

Coomassie blue staining of purified CTNS protein (Cat# TP313151). The protein was produced from HEK293T cells transfected with CTNS cDNA clone (Cat# RC213151) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC213151
• Predicted MW: 41.6 kDa
• Protein Sequence:
  Sequence Link

  >RC213151 representing NM_004937
  Red=Cloning site Green=Tags(s)
  
  MIRNWLTIFILFPLKLVEKCESSVSLTVPPVVKLENGSSTNVSLTLRPPLNATLVITFEITFRSKNITIL
  ELPDEVVVPPGVTNSSFQVTSQNVGQLTVYLHGNHSNQTGPRIRFLVIRSSAISIINQVIGWIYFVAWSI
  SFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSNDVFFSL
  HAVVLTLIIIVQCCLYERGGQRVSWPAIGFLVLAWLFAFVTMIVAAVGVITWLQFLFCFSYIKLAVTLVK
  YFPQAYMNFYYKSTEGWSIGNVLLDFTGGSFSLLQMFLQSYNNDQWTLIFGDPTKFGLGVFSIVFDVVFF
  IQHFCLYRKRPGYDQLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004928
• RefSeq Size: 2611
• RefSeq ORF: 1101
• Synonyms: CTNS-LSB; PQLC4; SLC66A4
• Locus ID: 1497
• UniProt ID: O60931, A0A0S2Z3K3
• Cytogenetics: 17p13.2
• Summary: 'This gene encodes a seven-transmembrane domain protein that functions to transport cystine out of lysosomes. Its activity is driven by the H+ electrochemical gradient of the lysosomal membrane. Mutations in this gene cause cystinosis, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]'
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome