PAF49 (CD3EAP) (NM_012099) Human Mass Spec Standard

CAT#: PH314394

CD3EAP MS Standard C13 and N15-labeled recombinant protein (NP_036231)

Product Images

Coomassie blue staining of purified CD3EAP protein (Cat# TP314394). The protein was produced from HEK293T cells transfected with CD3EAP cDNA clone (Cat# RC214394) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC214394
• Predicted MW: 54.8 kDa
• Protein Sequence:
  Sequence Link

  >RC214394 representing NM_012099
  Red=Cloning site Green=Tags(s)
  
  MEEPQAGDAARFSCPPNFTAKPPASESPRFSLEALTGPDTELWLIQAPADFAPECFNGRHVPLSGSQIVK
  GKLAGKRHRYRVLSSCPQAGEATLLAPSTEAGGGLTCASAPQGTLRILEGPQQSLSGSPLQPIPASPPPQ
  IPPGLRPRFCAFGGNPPVTGPRSALAPNLLTSGKKKKEMQVTEAPVTQEAVNGHGALEVDMALGSPEMDV
  RKKKKKKNQQLKEPEAAGPVGTEPTVETLEPLGVLFPSTTKKRKKPKGKETFEPEDKTVKQEQINTEPLE
  DTVLSPTKKRKRQKGTEGMEPEEGVTVESQPQVKVEPLEEAIPLPPTKKRKKEKGQMAMMEPGTEAMEPV
  EPEMKPLESPGGTMAPQQPEGAKPQAQAALAAPKKKTKKEKQQDATVEPETEVVGPELPDDLEPQAAPTS
  TKKKKKKKERGHTVTEPIQPLEPELPGEGQPEARATPGSTKKRKKQSQESRMPETVPQEEMPGPPLNSES
  GEEAPTGRDKKRKQQQQQPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_036231
• RefSeq Size: 3286
• RefSeq ORF: 1530
• Synonyms: ASE-1; ASE1; CAST; PAF49
• Locus ID: 10849
• UniProt ID: O15446
• Cytogenetics: 19q13.32
• Summary: Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. [UniProtKB/Swiss-Prot Function]
• Protein Families: Transcription Factors