HNRNPC (NM_031314) Human Mass Spec Standard

CAT#: PH315956

HNRNPC MS Standard C13 and N15-labeled recombinant protein (NP_112604)

Product Images

Coomassie blue staining of purified HNRNPC protein (Cat# TP315956). The protein was produced from HEK293T cells transfected with HNRNPC cDNA clone (Cat# RC215956) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC215956
• Predicted MW: 33.5 kDa
• Protein Sequence:
  Sequence Link

  >RC215956 representing NM_031314
  Red=Cloning site Green=Tags(s)
  
  MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIVGCSVHKGFAFVQYVNERNARAAVAGE
  DGRMIAGQVLDINLAAEPKVNRGKAGVKRSAAEMYGSVTEHPSPSPLLSSSFDLDYDFQRDYYDRMYSYP
  ARVPPPPPIARAVVPSKRQRVSGNTSRRGKSGFNSKSGQRGSSKSGKLKGDDLQAIKKELTQIKQKVDSL
  LENLEKIEKEQSKQAVEMKNDKSEEEQSSSSVKKDETNVKMESGGGADDSAEEGDLLDDDDNEDRGDDQL
  ELIKDDEKEAEEGEDDRDSANGEDDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_112604
• RefSeq Size: 3252
• RefSeq ORF: 918
• Synonyms: C1; C2; HNRNP; HNRPC; SNRPC
• Locus ID: 3183
• UniProt ID: P07910
• Cytogenetics: 14q11.2
• Summary: 'This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Spliceosome