QDPR (NM_000320) Human Mass Spec Standard

CAT#: PH317737

QDPR MS Standard C13 and N15-labeled recombinant protein (NP_000311)

Product Images

Coomassie blue staining of purified QDPR protein (Cat# TP317737). The protein was produced from HEK293T cells transfected with QDPR cDNA clone (Cat# RC217737) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC217737
• Predicted MW: 25.6 kDa
• Protein Sequence:
  Sequence Link

  >RC217737 representing NM_000320
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAGEARRVLVYGGRGALGSRCVQAFRARNWWVASVDVVENEEASASIIVKMTDSFTEQADQVTAE
  VGKLLGEEKVDAILCVAGGWAGGNAKSKSLFKNCDLMWKQSIWTSTISSHLATKHLKEGGLLTLAGAKAA
  LDGTPGMIGYGMAKGAVHQLCQSLAGKNSGMPPGAAAIAVLPVTLDTPMNRKSMPEADFSSWTPLEFLVE
  TFHDWITGKNRPSSGSLIQVVTTEGRTELTPAYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000311
• RefSeq Size: 1550
• RefSeq ORF: 732
• Synonyms: DHPR; HDHPR; PKU2; SDR33C1
• Locus ID: 5860
• UniProt ID: P09417, A0A140VKA9
• Cytogenetics: 4p15.32
• Summary: 'This gene encodes the enzyme dihydropteridine reductase, which catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin. This enzyme is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. Mutations in this gene resulting in QDPR deficiency include aberrant splicing, amino acid substitutions, insertions, or premature terminations. Dihydropteridine reductase deficiency presents as atypical phenylketonuria due to insufficient production of biopterin, a cofactor for phenylalanine hydroxylase. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways