Cytohesin 2 (CYTH2) (NM_017457) Human Mass Spec Standard

CAT#: PH317980

CYTH2 MS Standard C13 and N15-labeled recombinant protein (NP_059431)

Product Images

Coomassie blue staining of purified CYTH2 protein (Cat# TP317980). The protein was produced from HEK293T cells transfected with CYTH2 cDNA clone (Cat# RC217980) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC217980
• Predicted MW: 46.6 kDa
• Protein Sequence:
  Sequence Link

  >RC217980 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRK
  KFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNL
  VQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGL
  ERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRR
  WFILTDNCLYYFEYTTDKEPRGIIPQENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGN
  HMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_059431
• RefSeq Size: 4625
• RefSeq ORF: 1200
• Synonyms: ARNO; CTS18; CTS18.1; cytohesin-2; PSCD2; PSCD2L; SEC7L; Sec7p-L; Sec7p-like
• Locus ID: 9266
• UniProt ID: Q99418
• Cytogenetics: 19q13.33
• Summary: The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Protein Families: Druggable Genome