TFIIE alpha (GTF2E1) (NM_005513) Human Mass Spec Standard

CAT#: PH318763

GTF2E1 MS Standard C13 and N15-labeled recombinant protein (NP_005504)

Product Images

Coomassie blue staining of purified GTF2E1 protein (Cat# TP318763). The protein was produced from HEK293T cells transfected with GTF2E1 cDNA clone (Cat# RC218763) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC218763
• Predicted MW: 49.3 kDa
• Protein Sequence:
  Sequence Link

  >RC218763 representing NM_005513
  Red=Cloning site Green=Tags(s)
  
  MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDK
  FIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLE
  ANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTE
  IPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIW
  LRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAAN
  GDDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTP
  EEKEAYIAMGQRMFEDLFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005504
• RefSeq Size: 2969
• RefSeq ORF: 1317
• Synonyms: FE; TF2E1; TFIIE-A
• Locus ID: 2960
• UniProt ID: P29083
• Cytogenetics: 3q13.33
• Summary: ''
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors