GART (NM_175085) Human Mass Spec Standard

CAT#: PH320898

GART MS Standard C13 and N15-labeled recombinant protein (NP_780294)

Product Images

Coomassie blue staining of purified GART protein (Cat# TP320898). The protein was produced from HEK293T cells transfected with GART cDNA clone (Cat# RC220898) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC220898
• Predicted MW: 45.9 kDa
• Protein Sequence:
  Sequence Link

  >RC220898 representing NM_175085
  Red=Cloning site Green=Tags(s)
  
  MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFV
  VVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSA
  DFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVA
  PMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLT
  KNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTK
  GVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKD
  VGFRAIAFLQQPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_780294
• RefSeq Size: 2162
• RefSeq ORF: 1299
• Synonyms: AIRS; GARS; GARTF; PAIS; PGFT; PRGS
• Locus ID: 2618
• UniProt ID: P22102
• Cytogenetics: 21q22.11
• Summary: 'The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism