RAP1B (NM_001010942) Human Mass Spec Standard

CAT#: PH321793

RAP1B MS Standard C13 and N15-labeled recombinant protein (NP_001010942)

Product Images

Coomassie blue staining of purified RAP1B protein (Cat# TP321793). The protein was produced from HEK293T cells transfected with RAP1B cDNA clone (Cat# RC221793) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC221793
• Predicted MW: 20.6 kDa
• Protein Sequence:
  Sequence Link

  >RC221793 representing NM_001010942
  Red=Cloning site Green=Tags(s)
  
  MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDL
  YMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNN
  CAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001010942
• RefSeq Size: 2117
• RefSeq ORF: 552
• Synonyms: K-REV; RAL1B
• Locus ID: 5908
• UniProt ID: P61224, A0A024RB87
• Cytogenetics: 12q15
• Summary: 'This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011]'
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma