GIP (NM_004123) Human Mass Spec Standard

CAT#: PH322456

GIP MS Standard C13 and N15-labeled recombinant protein (NP_004114)

Product Images

Coomassie blue staining of purified GIP protein (Cat# TP322456). The protein was produced from HEK293T cells transfected with GIP cDNA clone (Cat# RC222456) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC222456
• Predicted MW: 17.1 kDa
• Protein Sequence:
  Sequence Link

  >RC222456 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVATKTFALLLLSLFLAVGLGEKKEGHFSALPSLPVGSHAKVSSPQPRGPRYAEGTFISDYSIAMDKIHQ
  QDFVNWLLAQKGKKNDWKHNITQREARALELAGQANRKEEEAVEPQSSPAKNPSDEDLLRDLLIQELLAC
  LLDQTNLCRLRSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004114
• RefSeq Size: 711
• RefSeq ORF: 459
• Synonyms: gastric inhibitory polypeptide; glucose-dependent insulinotropic polypeptide
• Locus ID: 2695
• UniProt ID: P09681
• Cytogenetics: 17q21.32
• Summary: 'This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome, Secreted Protein