PFKFB1 (NM_002625) Human Mass Spec Standard

CAT#: PH324599

PFKFB1 MS Standard C13 and N15-labeled recombinant protein (NP_002616)

Product Images

Coomassie blue staining of purified PFKFB1 protein (Cat# TP324599). The protein was produced from HEK293T cells transfected with PFKFB1 cDNA clone (Cat# RC224599) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC224599
• Predicted MW: 54.7 kDa
• Protein Sequence:
  Sequence Link

  >RC224599 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIG
  TPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERR
  SLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEE
  LDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRG
  KQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPHEQWKALNEIDAGVCEEMTYEEIQEHYPEEF
  ALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLH
  TVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002616
• RefSeq Size: 1756
• RefSeq ORF: 1413
• Synonyms: F6PK; HL2K; PFRX
• Locus ID: 5207
• UniProt ID: P16118
• Cytogenetics: Xp11.21
• Summary: 'This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]'
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism