NACA (NM_001113202) Human Mass Spec Standard

CAT#: PH325257

NACA MS Standard C13 and N15-labeled recombinant protein (NP_001106673)

Product Images

Coomassie blue staining of purified NACA protein (Cat# TP325257). The protein was produced from HEK293T cells transfected with NACA cDNA clone (Cat# RC225257) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC225257
• Predicted MW: 23.2 kDa
• Protein Sequence:
  Sequence Link

  >RC225257 representing NM_001113202
  Red=Cloning site Green=Tags(s)
  
  MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQS
  RSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAA
  EKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAI
  MELTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001106673
• RefSeq ORF: 645
• Synonyms: HSD48; NAC-alpha; NACA1; skNAC
• Locus ID: 4666
• UniProt ID: Q13765, A0A024RB41
• Cytogenetics: 12q13.3
• Summary: 'This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016]'
• Protein Families: Druggable Genome, Transcription Factors