RNASEH2B (NM_001142279) Human Mass Spec Standard

CAT#: PH326833

RNASEH2B MS Standard C13 and N15-labeled recombinant protein (NP_001135751)

Product Images

Coomassie blue staining of purified RNASEH2B protein (Cat# TP326833). The protein was produced from HEK293T cells transfected with RNASEH2B cDNA clone (Cat# RC226833) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC226833
• Predicted MW: 28.8 kDa
• Protein Sequence:
  Sequence Link

  >RC226833 representing NM_001142279
  Red=Cloning site Green=Tags(s)
  
  MAAGVDCGDGVGARQHVFLVSEYLKDASKKMKNGLMFVKLVNPCSGEGAIYLFNMCLQQLFEVKVFKEKH
  HSWFINQSVQSGGLLHFATPVDPLFLLLHYLIKADKEGKFQPLDQVVVDNVFPNCILLLKLPGLEKLLHH
  VTEEKGNPEIDNKKYYKYSKEKTLKWLEKKVNQTVAALKTNNVNVSSRVQSTAFFSGDQASTDKEEDYIR
  YAHGLISDYIPKELSDDLSKYLKLPEPSASLPNPPSKMAAQRQKRGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001135751
• RefSeq ORF: 771
• Synonyms: AGS2; DLEU8
• Locus ID: 79621
• UniProt ID: Q5TBB1
• Cytogenetics: 13q14.3
• Summary: RNase H2 is composed of a single catalytic subunit (A) and two non-catalytic subunits (B and C) and specifically degrades the RNA of RNA:DNA hybrids. The protein encoded by this gene is the non-catalytic B subunit of RNase H2, which is thought to play a role in DNA replication. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Aicardi-Goutieres syndrome type 2 (AGS2). [provided by RefSeq, Nov 2008]
• Protein Pathways: DNA replication