HTRA1 Mutant (NM_002775) Human Recombinant Protein

CAT#: TP700208

Recombinant protein of human HtrA serine peptidase 1 (HTRA1), S328A mutant, 20 ug

Product Images

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  An in vitro cleavage assay shows TSP1 is a direct substrate of HtrA1. TSP1 (500 ng) were incubated with the indicated amount of active HtrA1WT (WT) (OriGene TP322362) or inactive HtrA1S328A (S328A) (OriGene TP700208). The cleavage events were analyzed by immunoblotting. Filled triangle is the full-length form of TSP1 and HtrA1. Empty triangle is proteolysis products. TSP1 was probed using an antibody specific to the N-terminal domain. The molecular weight (kDa) reference is indicated in the left side of the image. Figure cited from Matrix Biol, PMID: 29572155

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  HtrA1 degrades preformed microtubules and avoids polymerization in vitro. Fluorescently labeled microtubules were polymerized in vitro and incubated with S328A HtrA1 (OriGene, TP700208) (center) and HtrA1 (OriGene, TP322362) (right). Images were acquired at 5 minutes, 6 hours, and 24 hours after adding HtrA1. Scale bar, 10 um. Figure cited from EBioMedicine, PMID: 29269042

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: A DNA sequence from TrueORF clone, RC222362 with a T982G mutation, encoding the mutant form (S328A) of human HTRA
• Tag: C-Myc/DDK
• Predicted MW: 49 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: Protein degradation enzyme (PMID: 29269042)
  Enzyme activity (PMID: 29572155)
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_002766
• Locus ID: 5654
• Cytogenetics: 10q26.13
• Refseq Size: 2133
• Refseq ORF: 443
• Synonyms: ARMD7; CADASIL2; CARASIL; HtrA; L56; ORF480; PRSS11
• Summary: 'This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome, Protease, Secreted Protein