Isocitrate dehydrogenase (IDH1) Mutant (NM_005896) Human Recombinant Protein

CAT#: TP710051

Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132S) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells


  View other "IDH1" proteins (19)

USD 425.00

In Stock*

Size
    • 20 ug

Product Images

Other products for "IDH1"

Specifications

Product Data
Species Human
Expression Host Sf9
Expression cDNA Clone or AA Sequence Two DNA sequences from TrueORF clone, RC210582 and RC400099, respectively encoding human full-length IDH1 and Mutant IDH1(R132S)
Tag C-DDK
Predicted MW 47 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 50mM Tris-HCl pH8.0, 150mM NaCl, 10%glycerol
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq NP_005887
Locus ID 3417
Cytogenetics 2q34
Refseq Size 2339
Refseq ORF 1245
Synonyms HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Summary 'Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]'
Protein Pathways Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

Documents

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.