Dead Cas9 Staphylococcus aureus Protein

CAT#: TP790184

Purified recombinant protein of mutant(D10A/H840A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- terminal of the protein, with N-terminal HIS tag, expressed in E. coli


USD 125.00

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Size
    • 500 pmol

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Specifications

Product Data
Species Staphylococcus aureus
Expression Host E. coli
Expression cDNA Clone or AA Sequence MHHHHHHENL YFQGAIAPKK KRKVEDPKKK RKVDMDKKYS IGLAIGTNSV
GWAVITDEYK VPSKKFKVLG NTDRHSIKKN LIGALLFDSG ETAEATRLKR
TARRRYTRRK NRICYLQEIF SNEMAKVDDS FFHRLEESFL VEEDKKHERH
PIFGNIVDEV AYHEKYPTIY HLRKKLVDST DKADLRLIYL ALAHMIKFRG
HFLIEGDLNP DNSDVDKLFI QLVQTYNQLF EENPINASGV DAKAILSARL
SKSRRLENLI AQLPGEKKNG LFGNLIALSL GLTPNFKSNF DLAEDAKLQL
SKDTYDDDLD NLLAQIGDQY ADLFLAAKNL SDAILLSDIL RVNTEITKAP
LSASMIKRYD EHHQDLTLLK ALVRQQLPEK YKEIFFDQSK NGYAGYIDGG
ASQEEFYKFI KPILEKMDGT EELLVKLNRE DLLRKQRTFD NGSIPHQIHL
GELHAILRRQ EDFYPFLKDN REKIEKILTF RIPYYVGPLA RGNSRFAWMT
RKSEETITPW NFEEVVDKGA SAQSFIERMT NFDKNLPNEK VLPKHSLLYE
YFTVYNELTK VKYVTEGMRK PAFLSGEQKK AIVDLLFKTN RKVTVKQLKE
DYFKKIECFD SVEISGVEDR FNASLGTYHD LLKIIKDKDF LDNEENEDIL
EDIVLTLTLF EDREMIEERL KTYAHLFDDK VMKQLKRRRY TGWGRLSRKL
INGIRDKQSG KTILDFLKSD GFANRNFMQL IHDDSLTFKE DIQKAQVSGQ
GDSLHEHIAN LAGSPAIKKG ILQTVKVVDE LVKVMGRHKP ENIVIEMARE
NQTTQKGQKN SRERMKRIEE GIKELGSQIL KEHPVENTQL QNEKLYLYYL
QNGRDMYVDQ ELDINRLSDY DVDAIVPQSF LKDDSIDNKV LTRSDKNRGK
SDNVPSEEVV KKMKNYWRQL LNAKLITQRK FDNLTKAERG GLSELDKAGF
IKRQLVETRQ ITKHVAQILD SRMNTKYDEN DKLIREVKVI TLKSKLVSDF
RKDFQFYKVR EINNYHHAHD AYLNAVVGTA LIKKYPKLES EFVYGDYKVY
DVRKMIAKSE QEIGKATAKY FFYSNIMNFF KTEITLANGE IRKRPLIETN
GETGEIVWDK GRDFATVRKV LSMPQVNIVK KTEVQTGGFS KESILPKRNS
DKLIARKKDW DPKKYGGFDS PTVAYSVLVV AKVEKGKSKK LKSVKELLGI
TIMERSSFEK NPIDFLEAKG YKEVKKDLII KLPKYSLFEL ENGRKRMLAS
AGELQKGNEL ALPSKYVNFL YLASHYEKLK GSPEDNEQKQ LFVEQHKHYL
DEIIEQISEF SKRVILADAN LDKVLSAYNK HRDKPIREQA ENIIHLFTLT
NLGAPAAFKY FDTTIDRKRY TSTKEVLDAT LIHQSITGLY ETRIDLSQLG
GDPKKKRKVE DPKKKRKVDT R
Tag N-His
Predicted MW 164.5 kDa
Concentration 20uM
Purity > 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer 10mM Tris,300mM NaCl,0.1mM EDTA,1mM DTT, pH7.4, 50% glycerol.
Endotoxin < 0.5 EU per 1 µg of the protein by the LAL
Storage Store at -20℃ after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data

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