Filamin Chicken Protein

CAT#: BA1010

Filamin chicken protein, 0.25 mg


USD 280.00

2 Weeks*

Size
    • 250 ug

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Specifications

Product Data
Species Chicken
Protein Source Gizzard
Predicted MW 250 kDa
Concentration lot specific
Purity >90% > 90% (determined by SDS gelelectrophoresis)
Buffer Presentation State: Purified
State: Lyophilized
Buffer System: 20 mM Tris/acetate buffer pH 7.6, 0.1mM EDTA, 2 mM DTT, 20 mM NaCl
Reconstitution Restore with 250 µl Laemmli buffer (final volume 250 µl) or 9M urea buffer (e.g. IEF or NEPHGE buffer) Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room temperature, separate insoluble rests by a short centrifugation step.
Preparation Lyophilized
Applications Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protein Description Filamin, purified polypeptide
Note Isoelectric Point: pI 6.4
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -70°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.