GFAP Bovine Protein

CAT#: BA1011

GFAP bovine protein, 0.25 mg

Product Images

Specifications

Product Data

• Species: Bovine
• Protein Source: Spinal Cord
• Predicted MW: 52 kDa
• Concentration: lot specific
• Purity: >98% (determined by SDS gelelectrophoresis).
• Buffer: Presentation State: Purified
  State: Lyophilized
  Buffer System: Final Solution contains 10mM Sodium Phosphate buffer pH 7.5, 6M Urea, 2mM DTT, 1 mM EDTA, 10mM Methylammonium Chloride
• Reconstitution: Restore with distilled water.
  BA1011S: 80 µl (final volume 100 µl).
  BA1011 : 200 µl (final volume 250 µl).
• Preparation: Lyophilized
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
  Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
  For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
• Protein Description: Bovine Glial Filament Protein (GFP)
• Note: Isoelectric Point: pI 5.4
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the protein undiluted at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_776490
• Locus ID: 281189
• Summary: glial fibrillary acidic protein