Bovine ECGFpro1 + Heparin (complete for blood ECs) Protein

CAT#: DA3560

Bovine ECGFpro1 + Heparin (complete for blood ECs) protein, 6 mg

Specifications

Product Data

• Protein Source: Brain
• Buffer: State: Lyophilized, Freeze dried powder (Freeze dried)
  Buffer System: H2O
  Preservative: None
• Bioactivity: Specific: Bovine ECGFpro1 is effective on mouse, bovine and human cells.
• Reconstitution: Endothelial cell growth factor is supplied as a sterile lyophilized powder containing 6 mg protein per vial. To obtain a stock solution reconstitute the contents of the vial in 2 ml of prewarmed (37 °C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. Although the stock solution can be added aseptically to sterile tissue culture medium, it is recommended that medium containing diluted product is aseptically filtered prior to use.
  The ECGF + VEGF-A are sufficient for 500 ml growth medium.
• Preparation: Lyophilized, Freeze dried powder (Freeze dried)
• Protein Description: Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml, optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.
• Storage: Prior to reconstitution store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Following reconstitution store at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.