Streptavidin (Beta-Galactosidase conjugated) Protein

CAT#: RA021GAL

Streptavidin (Beta-Galactosidase conjugated) protein, 1 mg

Specifications

Product Data

• Protein Source: S. avidinii
• Concentration: lot specific
• Purity: Prepared from electrophoretically pure Streptavidin.
• Buffer: Presentation State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction.
  Buffer System: 0.02 M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, 0.01 M Magnesium Chloride and 0.01 M Beta-Mercaptoethanol, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Beta-Galactosidase (E. coli, MW 540,000 daltons)
• Preparation: Liquid (sterile filtered) purified Ig fraction.
• Applications: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
  Recommended Dilutions:
  ELISA: 1/8000-1/32000.
  Western Blot: 1/500-1/2500.
  Immunohistochemistry: 1/200-1/1000.
• Note: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- beta galactosidase (E.coli) and anti-Streptavidin.
  No reaction was observed against anti-Avidin.
• Storage: Store vial at 2-8°C before opening.
  DO NOT FREEZE!
  This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  Freezing beta galactosidase conjugates will result in a substantial loss of enzymatic activity.
• Stability: Shelf life: one year from despatch.