Streptavidin Protein

CAT#: RA021HRP

Streptavidin protein, 1 mg

Specifications

Product Data

• Protein Source: S. avidinii
• Concentration: lot specific
• Purity: Prepared from Chromatographically purified streptavidin and labelled with Horseradish Peroxidase (HRP).
• Buffer: State: Lyophilized purifed protein.
  Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: Horseradish Peroxidase (HRP)
  Preservative: 0.01% (w/v) Gentamicin sulfate
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Presentation Label: HRP
• Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purifed protein.
• Applications: Suitable for Immunoblotting (Western or Dot blot, 1/10,000-1/40,000), ELISA (1/20,000-1/200,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-biotin-avidin based enzymatic assays.
• Note: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.
  
  Caution: Do Not Add Sodium Azide
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.