Streptavidin Protein

CAT#: RA021P

Streptavidin protein, 5 mg

Specifications

Product Data

• Protein Source: S. avidinii
• Purity: : This product is chromatographically pure Streptavidin and shows predominantly a single 53,600 dalton band by SDS-PAGE.
  Lane 1 - molecular weight markers, Lane 2, Lot 15243 Streptavidin.
• Buffer: Presentation State: Purified
  State: Lyophilized.
  Buffer System: 0.15 M Sodium Chloride without preservatives or stabilizers.
• Bioactivity: Specific: 16.3 U/mg protein (biotin titration method)
• Reconstitution: Restore with deionized water (or equivalent).
• Preparation: Lyophilized.
• Applications: Suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.
• Protein Description: Purified Streptavidin is a protein composed of 4 essentially identical polypeptide chains (homotetramer). It contains no cysteine residues, carbohydrate side chains or associated cofactors.
• Note: Characteristics: Different preparations of streptavidin show considerable heterogeneity at both the amino- and carboxy-termini of each subunit polypeptide due to proteolysis during biosynthesis and secretion. Monomeric subunits of streptavidin are synthesized as 183 amino acid prepeptides. During secretion by Streptomyces sp., a 24 amino acid leader sequence is cleaved from these polypeptides resulting in newly secreted monomers of 159 amino acids (Agarana et al, 1986). Upon longer incubations in culture these monomers are progressively cleaved to "core" subunits containing 125-127 amino acids (Bayer et al, 1989; Hendrickson et al, 1989). Preparations of streptavidin are relatively stable over a wide pH range and extremely heat stable, requiring up to 20 minutes at 100°C in 0.2% SDS to dissociate the subunits (Bayer et al, 1986). Strong chaotropic agents such as 6 M urea have been reported to dissociate the streptavidin tetramer into dimers (Sano and Cantor, 1990). These dimers appear to be stable in urea without appearance of monomers. Unproteolyzed and proteolyzed preparations of streptavidin appear to bind biotin with equal affinity. The most highly proteolyzed tetramers may bind over 16 micrograms of d-biotin per milligram of protein. Bayer (1989) reports that biotinylated enzymes bind most effectively to truncated streptavidin in ELISA-type assays.
  The dissociation constant for biotin is approximately 10-15 M (Chaiet et al, 1964). The formation of the streptavidin-biotin complex is stable over wide pH and temperature ranges. The complex is generally disrupted only by conditions which lead to irreversible denaturation of the protein. Analogs of biotin such as 2-imino-biotin bind reversibly to the protein with complex formation at high pH (> 9.5) and dissociation at low pH (< 4) (Bayer et al, 1986; Hofmann et al, 1980).
  The extinction coefficient of streptavidin is E(0.1% at 280 nm): 3.17 (Suter et al,1988)
  Biotin Binding: The biotin-binding activity of streptavidin is determined using a modification of the dye-binding assay of Green (1970). One unit will bind one microgram of d-biotin at pH 7.0.
  Suggestions for Use: Bayer (1989) reports that streptavidin may form aggregates under certain conditions. Streptavidin is highly soluble under alkaline conditions (pH > 8.5). Streptavidin is often supplied lyophilized. Under these conditions there is a tendency for the material to aggregate if it is redissolved in water or other low ionic strength buffers at neutral or acidic pH. As a convenience to customers, Streptavidin has been lyophilized from a dilute sodium chloride solution at mildly alkaline pH. This material is readily soluble in water. The activity of the material recovered after reconstitution under these conditions is undiminished. We recommend dissolving streptavidin in de-ionized water or, preferably, 1.0 mM sodium bicarbonate buffer (pH 9) at twice the desired final protein concentration. The protein may then be diluted with an equal volume of 2x buffer to produce a stock solution. Upon standing some turbidity may develop in certain buffers. Centrifugation will usually yield a clear solution with negligible loss of streptavidin.
• Storage: Store vial at 2-8°C prior to restoration.
  Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.