Porcine IgM Porcine Protein
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Specifications
Product Data | |
Species | Porcine |
Protein Source | Serum |
Concentration | lot specific |
Purity | >95% pure. |
Buffer | State: Liquid (sterile filtered) purified Ig fraction. Buffer System: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0, with 0.09% (w/v) Sodium Azide as preservative. |
Preparation | Liquid (sterile filtered) purified Ig fraction. |
Applications | Swine IgM whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.Suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins. |
Note | This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine Serum and anti-Swine IgM (µ chain specific). No reaction was observed against anti-Swine IgG F(c). Some light chain cross reactivity will occur with anti-Swine IgG. |
Storage | Store vial at 2-8°C prior to opening. This product is stable at 2-8°C as an undiluted liquid for one month. Dilute only prior to immediate use. For extended storage mix liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data |
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