Avidin Chicken Protein

CAT#: RA103F

Avidin chicken protein, 2 mg

Specifications

Product Data

• Species: Chicken
• Protein Source: Egg white
• Concentration: lot specific
• Purity: (Prepared from chromatographical pure Avidin).
• Buffer: State: Lyophilized
  Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (Immunoglobulin and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
  Absorption/Emission: 495 nm / 528 nm
  Fluorochrome/Protein Ratio: 2.0 moles FITC per mole of Avidin
  Presentation Label: FITC
• Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
  For extended storage mix product with glycerol to 50%.
• Preparation: Lyophilized
• Applications: This product is designed for Immunofluorescence microscopy, fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA. 1/10,000-1/50,000
  Immunofluorescence: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.
• Protein Description: Avidin Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Avidin. No reaction was observed against anti-Streptavidin.
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the protein undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_990651
• Locus ID: 396260
• Summary: avidin