Mouse IgG (Fc specific) Mouse Protein

CAT#: RA106

Mouse IgG (Fc specific) mouse protein, 1 mg

Specifications

Product Data

• Species: Mouse
• Protein Source: Serum
• Concentration: lot specific
• Purity: This product was prepared from normal serum Delipidation, Salt Fractionation, Ion Exchange Chromatography followed by papain Digestion and extensive dialysis against the buffer
• Buffer: State: Lyophilized purified protein
  Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester.
  Biotin/Protein Ratio: 10-20 BAC molecules per Mouse IgG F(c) molecule.
  Preservative: 0.01% (w/v) Sodium Azide
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Presentation Label: Biotin
• Reconstitution: Restore with 1.0 ml of deionized water(or equivalent)
• Preparation: Lyophilized purified protein
• Applications: Immunoblotting.
  ELISA.
  Immunohistochemistry.
  Immunomicroscopy.
  as well as other antibody based assays using streptavidin or avidin conjugates.
• Protein Description: Mouse IgG F(c) fragment Biotin conjugated.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum.
  No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain.
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.