VEGF Receptor 2 (KDR) (NM_002253) Human Over-expression Lysate

CAT#: LC400826

KDR HEK293T cell transient overexpression lysate (as WB positive control)

Product Images

Western blot validation of overexpression lysate (Cat# LY400826) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219851 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Applications: WB
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC219851
• Tag: C-Myc/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Accession Number: NM_002253, NP_002244
• Other Names: CD309; FLK1; VEGFR; VEGFR2
• Predicted MW: 152 kDa
• Components: 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
• Storage: The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
• Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.

Reference Data

• RefSeq: NP_002244
• Locus ID: 3791
• Cytogenetics: 4q12
• Protein Families: WB
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion, VEGF signaling pathway