ATP6V1E1 (NM_001696) Human Over-expression Lysate
CAT#: LC419796
ATP6V1E1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
Specifications
Product Data | |
Species | Human |
Applications | WB |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC201267 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_001696, NP_001687 |
Other Names | ARCL2C; ATP6E; ATP6E2; ATP6V1E; P31; Vma4 |
Predicted MW | 26.1 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_001687 |
Locus ID | 529 |
Cytogenetics | 22q11.21 |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC422039 | ATP6V1E1 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LC425635 | ATP6V1E1 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LY419796 | Transient overexpression lysate of ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1 |
USD 396.00 |
|
LY422039 | Transient overexpression lysate of ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 3 |
USD 396.00 |
|
LY425635 | Transient overexpression lysate of ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 3 |
USD 396.00 |
|
PH301267 | ATP6V1E1 MS Standard C13 and N15-labeled recombinant protein (NP_001687) |
USD 2,055.00 |
|
TP301267 | Recombinant protein of human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1 |
USD 823.00 |
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