MASP2 (NC_006610) Human Over-expression Lysate

CAT#: LC434596

Transient overexpression lysate of MASP2, a key intermediate in over-activation of lectin complement pathway

Product Images

Western blot validation of overexpression lysate (LC434596) using an anti-DDK antibody (TA50011-100) and anti-MASP1 antibody (TA812530). Lane 1:Molecular Wt. Standard. Lane 2 & 3: Cell lysates from HEK293T cells transfected with RC216665 using transfection reagent MegaTran 2.0 (TT210002) and stained with antibodies as stated.

Specifications

Product Data

• Species: Human
• Applications: WB
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC216665
• Tag: C-MYC/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Accession Number: NC_006610, NP_006601.2
• Predicted MW: 75.69 kDa
• Components: 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
• Storage: The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
• Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation, the products are supplied as lyophilized proteins.

Reference Data

• RefSeq: NP_006601.2
• Protein Families: WB
• Protein Pathways: Complement and coagulation cascades