alpha Adducin (ADD1) (NM_176801) Human Over-expression Lysate

CAT#: LY406127

Transient overexpression lysate of adducin 1 (alpha) (ADD1), transcript variant 4

Specifications

Product Data

• Species: Human
• Applications: WB
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC214897
• Tag: C-Myc/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Accession Number: NM_176801, NP_789771
• Other Names: ADDA
• Predicted MW: 73.2 kDa
• Components: 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
• Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).

Reference Data

• RefSeq: NP_789771
• Locus ID: 118
• Cytogenetics: 4p16.3
• Protein Families: WB