CUL4A (NM_003589) Human Over-expression Lysate
CAT#: LY429154
Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 2
Size: 20 ug
Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC203463 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_003589 |
Predicted MW | 87.7 kDa |
Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT) |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping. |
Reference Data | |
RefSeq | NP_003580 |
Locus ID | 8451 |
Cytogenetics | 13q34 |
Protein Pathways | Nucleotide excision repair, Ubiquitin mediated proteolysis |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC400388 | CUL4A HEK293T cell transient overexpression lysate (as WB positive control) |
USD 155.00 |
|
LC418559 | CUL4A HEK293T cell transient overexpression lysate (as WB positive control) |
USD 99.00 |
|
LC429154 | CUL4A HEK293T cell transient overexpression lysate (as WB positive control) |
USD 99.00 |
|
LY400388 | Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 1 |
USD 495.00 |
|
LY418559 | Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 2 |
USD 325.00 |
|
PH303463 | CUL4A MS Standard C13 and N15-labeled recombinant protein (NP_003580) |
USD 2,055.00 |
|
TP303463 | Purified recombinant protein of Homo sapiens cullin 4A (CUL4A), transcript variant 2 |
USD 867.00 |
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