VEGF-A (His-tag) Mouse Protein

CAT#: AR09272PU-L

VEGF-A (His-tag) mouse recombinant protein, 0.25 mg

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
• Tag: His-tag
• Predicted MW: 16.3 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
• Bioactivity: Biological: Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml.
  Activity Assay
  1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast )
  2. Maintenance Condition: RPMI 1640 containing 10% FBS
  3. Assay medium: serum free RPMI 1640
  4. Cell density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
  5. Incubation time: 24 hr (after sample treatment)
  6. Concentration range: 1 pg/ml – 1 ug/ml
  7. Detection method : BrdU assay
• Preparation: Liquid purified protein
• Protein Description: Recombinant mouse VEGF-A protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001020421
• Locus ID: 22339
• UniProt ID: Q00731, A0A1L1SVG2
• Cytogenetics: 17 22.79 cM
• Synonyms: Vegf; Vpf
• Summary: This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]