Vimentin Bovine Protein

CAT#: AR09359PU-N

Vimentin bovine protein, 0.25 mg

Product Images

Specifications

Product Data

• Species: Bovine
• Predicted MW: 57,000
• Concentration: lot specific
• Purity: >98% determined by SDS gelelectrophoresis
• Buffer: Presentation State: Purified
  State: Lyophilised purified protein
  Buffer System: 10 mM sodium phosphate pH 7.5, 6M urea, 2 mM DTT, 1 mM EDTA, 10 mM methylammonium chloride
• Reconstitution: Reconstitute with 200 µl distilled water (final volume 250 µl)
  Reconstitute with 80 µl distilled water (final volume 100 µl)
• Preparation: Lyophilised purified protein
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
  Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Protein Description: Bovine vimentin
• Note: Isoelectric Point: pI 5.3
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_776394
• Locus ID: 280955
• Cytogenetics: 13q16-q17
• Summary: vimentin