Adenylate kinase 1 / AK1 (1-194, His-tag) Human Protein

CAT#: AR09708PU-L

Adenylate kinase 1 / AK1 (1-194, His-tag) human recombinant protein, 0.5 mg


USD 845.00

2 Weeks*

Size
    • 500 ug

Product Images

Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK
Tag His-tag
Predicted MW 23.7 kDa
Concentration lot specific
Purity >95%
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol
Bioactivity Specific: > 6.0 units/ml.
One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25°C.
Preparation Liquid purified protein
Applications Protocol: Activity Assay
1. Prepare 1.4 ml assay buffer (Assay buffer: 58 mM glycylglycine, 2.0 mM adenosine 5'-diphosphate, 2.3 mM beta-nicotinamide adenine dinucleotide phosphate, 10 mM magnesium chloride, 10 mM glucose, 2 unit hexokinase, 1 unit glucose-6-phosphate dehydrogenase, 0.003% (w/v) BSA, pH 7.5)
- G-6-PDH/Hex (sigma,Cat.No. H-8629)
2. Add 50 ul of recombinant AK1 protein with various concentrations (0.5ug, 1ug) and read the increase in A340nm for 5 minutes.
Protein Description Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_000467
Locus ID 203
UniProt ID P00568, Q6FGX9
Cytogenetics 9q34.11
Synonyms HTL-S-58j
Summary 'This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]'
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Purine metabolism

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.