CD86 (Fc portion) Human Protein

CAT#: AR31193PU-N

CD86 (Fc portion) human recombinant protein, 0.1 mg

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  LKIQAYFNET ADLPCQFANS QNQSLSELVV FWQDQENLVL NEVYLGKEKF DSVHSKYMGR TSFDSDSWTL RLHNLQIKDK GLYQCIIHHK KPTGMIRIHQ MNSELSVLAN FSQPEIVPIS NITENVYINL TCSSIHGYPE PKKMSVLLRT KNSTIEYDGV MQKSQDNVTE LYDVSISLSV SFPDVTSNMT IFCILETDKT RLLSSPFSIE LEDPQPPPDH GGPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK
• Predicted MW: 52.2 kDa
• Purity: >95% pure by SDS-PAGE and HPLC analyses
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
• Bioactivity: Biological:
  Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 cells. The expected ED₅₀ for this effect is 0.5-1.5 μg/ml.
• Endotoxin: < 0.1 ng per µg (1EU/µg)
• Reconstitution: Restore in Water to a concentration of 0.1-1.0 mg/ml.
  This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
• Preparation: Lyophilized purified protein
• Protein Description: Recombinant Human B7-2Fc is a homodimeric B7-2 fusion protein, whose monomer contains a total of 453 amino acid residues, consisting of 222 amino acid residues corresponding to the extracellular domain of Human B7-2, fused to the Fc portion of Human IgG1.
  The calculated molecular weight of the B7-2 Fc monomer is 51.2 kDa.
• Note: Centrifuge the vial prior to opening!
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001193853
• Locus ID: 942
• UniProt ID: P42081, A8K632
• Cytogenetics: 3q13.33
• Synonyms: B7-2; B7.2; B70; CD28LG2; LAB72
• Summary: 'This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]'
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis