PCBP1 (1-163, His-tag) Human Protein

CAT#: AR51138PU-N

PCBP1 (1-163, His-tag) human recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC
• Tag: His-tag
• Predicted MW: 19.6 kDa
• Concentration: lot specific
• Purity: >85% by SDS - PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Preparation: Liquid purified protein
• Protein Description: Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_006187
• Locus ID: 5093
• UniProt ID: Q15365, Q53SS8
• Cytogenetics: 2p13.3
• Synonyms: HEL-S-85; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX
• Summary: 'This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Spliceosome