BMP2 / BMP2A Human Protein

CAT#: DA3502X

BMP2 / BMP2A human recombinant protein, 25 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 26 kDa
• Purity: >95% pure by SDS-PAGE and visualised by silver stain
• Buffer: Presentation State: Purified
  State: Lyophilized purified Ig fraction
  Buffer System: 50mM Acetic Acid
  Stabilizer: None
• Bioactivity: Biological: Measured by the ability of BMP-2 to induce Alkaline Phosphatase production by C2C12 myogenic cells.
  The ED50 for this effect is typically 0.3-0.8 µg/ml.
  Specific: 2.5 x 10³ units/mg
• Endotoxin: < 0.1 ng per µg of BMP-2
• Reconstitution: The lyophilized BMP-2 is best soluble in 50 mM Acetic Acid and at a concentration of 0.1 mg/ml but should be also soluble in most aqueous buffers when the pH is below 6.0
• Preparation: Lyophilized purified Ig fraction
• Protein Description: Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein.
  BMP-2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity.
  The amino acid sequence of recombinant Human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector.
• Storage: Lyophilized samples are stable for 6 months from despatch at -20°C to -70°C.
  Reconstituted BMP-2 should be stored in working aliquots at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Stable for at least 3 months from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001191
• Locus ID: 650
• UniProt ID: P12643, C8C060
• Cytogenetics: 20p12.3
• Synonyms: BDA2; BMP2A; SSFSC
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]'
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway