Hepatocyte growth factor / HGF Human Protein

CAT#: DA3506

Hepatocyte growth factor / HGF human recombinant protein, 5 µg

Specifications

Product Data

• Species: Human
• Expression Host: Insect
• Predicted MW: 78 kDa
• Concentration: lot specific
• Purity: >95% pure by SDS-PAGE and visualised by silver stain.
• Buffer: Presentation State: Purified
  State: Lyophilized without preservatives.
  Buffer System: 50 mM Acetic Acid with BSA as stabilizer.
• Bioactivity: Biological: Human HGF is fully biologically active when compared to standards.
  The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 0.5-1.0 ng/ml.
• Endotoxin: < 0.1 ng per µg of HGF
• Reconstitution: The lyophilized human HGF is soluble in water and most aqueous buffers.
  It can be restored in water to a concentration of 100 µg/ml. Further dilutions should be made into buffer containing protein or medium containing serum.
• Preparation: Lyophilized without preservatives.
• Protein Description: Recombinant human HGF is a 78.0 kDa protein.
• Storage: The lyophilized protein is stable at -20°C for one year from despatch.
  Reconstituted human HGF is stable for three months when stored in working aliquots
  at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Stable for at least 3 months from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_000592
• Locus ID: 3082
• UniProt ID: P14210
• Cytogenetics: 7q21.11
• Synonyms: DFNB39; F-TCF; HGFB; HPTA; SF
• Summary: 'This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015]'
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma