VEGF-C / Flt4-L (VEGF-C152S) Rat Protein
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Specifications
Product Data | |
Species | Rat |
Expression Host | Insect |
Predicted MW | 18-24 kDa |
Purity | >90% by SDS-PAGE and visualised by silver stain |
Buffer | Presentation State: Purified State: Lyophilized protein Buffer System: 50 mM Acetic Acid Stabilizer: BSA |
Bioactivity | Biological: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT-4 cells). The ED50 for this effect is typically 150-300 ng/ml. |
Endotoxin | < 0.1 ng per µg of VEGF-C152S |
Reconstitution | The lyophilized VEGF-C152S is soluble in water and most aqueous buffers. Restore in PBS or medium to a concentration not lower than 50 µg/ml. |
Preparation | Lyophilized protein |
Protein Description | VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C152S is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_446105 |
Locus ID | 114111 |
UniProt ID | O35757 |
Cytogenetics | 16p11 |
Summary | platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006] |
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