VEGF-C / Flt4-L (VEGF-C152S) Rat Protein

CAT#: DA3520X

VEGF-C / Flt4-L (VEGF-C152S) rat recombinant protein, 20 µg


USD 290.00

2 Weeks*

Size
    • 20 ug

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Specifications

Product Data
Species Rat
Expression Host Insect
Predicted MW 18-24 kDa
Purity >90% by SDS-PAGE and visualised by silver stain
Buffer Presentation State: Purified
State: Lyophilized protein
Buffer System: 50 mM Acetic Acid
Stabilizer: BSA
Bioactivity Biological: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT-4 cells). The ED50 for this effect is typically 150-300 ng/ml.
Endotoxin < 0.1 ng per µg of VEGF-C152S
Reconstitution The lyophilized VEGF-C152S is soluble in water and most aqueous buffers. Restore in PBS or medium to a concentration not lower than 50 µg/ml.
Preparation Lyophilized protein
Protein Description VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C152S is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_446105
Locus ID 114111
UniProt ID O35757
Cytogenetics 16p11
Summary platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.