Flt3 ligand Human Protein

CAT#: DA3543

Flt3 ligand human protein, 10 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGG LWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP P SCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSS TLP PPWSPRPLEA TAPTA
• Predicted MW: 17.6 kDa
• Purity: >98% by RP-HPLC, Anion-exchange FPLC, Silverstain
• Buffer: Presentation State: Purified
  State: Lyophilized (freeze-dried) without stabilizer
• Bioactivity: Biological: Human rh Flt3-ligand is fully biologically active when compared to standards.
  The ED50 range is 0.5-1.0 ng/ml corresponding to a specific activity of 1-2MUnits/mg, calculated by the dose-dependant stimulation of the proliferation of Human OCMI-AML5 cells.
• Endotoxin: < 0.1 ng per µg (IEU/µg) of rh Flt3-Ligand
• Reconstitution: Recombinant Flt3-Ligand is soluble in water and most aqueous buffers.
  Restore in water to a concentration not less than 0.1 mg/ml.
  This solution can then be stored at 2-8°C for future use or diluted into other buffered solutions. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
• Preparation: Lyophilized (freeze-dried) without stabilizer
• Protein Description: Flt3-ligand produced in E. coli is a soluble non-glycosylated 17.6 kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001191431
• Locus ID: 2323
• UniProt ID: P49771, B7ZLY4
• Cytogenetics: 19q13.33
• Synonyms: FL; FLG3L; FLT3L
• Summary: 'Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]'
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer