Adiponectin (biologically active) Mouse Protein

CAT#: PA502X

Adiponectin (biologically active) mouse protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK
• Concentration: lot specific
• Purity: >98% as determined by densitometric image analysis
• Buffer: Presentation State: Purified
  State: Lyophilized (0.4µm filtered) purified protein
  Buffer System: 0.05M Phosphate buffer, 0.075M NaCl, pH 7.4
• Bioactivity: Biological: Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocyte. It can also activate AMPK in HepG2 human hepatocytes at the concentration of as low as 1.0 µg/ml. In vitro gluconeogenesis assay in primary rat hepatocytes was performed, showing the murine adiponectin derived from mammalian cells can inhibit glucose production.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
• Preparation: Lyophilized (0.4µm filtered) purified protein
• Applications: ELISA.
  Western Blot.
  Cell culture and/or animal studies.
• Protein Description: Mouse Adiponectin
• Note: Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
  Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_033735
• Locus ID: 11450
• UniProt ID: Q60994
• Cytogenetics: 16 13.96 cM
• Synonyms: 30kDa; Acdc; Acrp30; Ad; Adid; adipo; apM1; APN; GBP28