Protein A (FITC conjugated) Staphylococcus aureus Protein

CAT#: RA100F

Protein A (FITC conjugated) s. aureus protein, 5 mg

Specifications

Product Data

• Species: Staphylococcus aureus
• Concentration: lot specific
• Purity: This product was prepared from chromatographically pure Protein A.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.
• Buffer: State: Lyophilized purified Ig fraction.
  Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons).
  Absorption/Emission Wavelength: 495 nm/528 nm.
  Fluorochrome/Protein Ratio: 4.6 moles FITC per mole of Protein A.
  Presentation Label: FITC
• Reconstitution: Restore with 5.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purified Ig fraction.
• Applications: Immunofluorescence Microscopy (1/1,000-1/5,000)
  Flow Cytometry/FACS (1/1,000-1/5,000) as well as other antibody based fluorescent assays.
• Protein Description: Fluorescein Conjugated Protein A.
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as undiluted liquid. Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.