Protein A (Sepharose) Staphylococcus aureus Protein
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Specifications
Product Data | |
Species | Staphylococcus aureus |
Concentration | lot specific |
Buffer | State: Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads). Buffer System: 20% (v/v) Ethanol Label: Sepharose (TM) Presentation Label: Sepharose |
Bioactivity | Specific: ~35 mg Human IgG bound per cc of drained beads. |
Preparation | Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads). |
Applications | Ideal for laboratory and process scale recovery and purification of monoclonal antibodies from dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 ul per immunoprecipitation reaction |
Protein Description | Native Protein A (Staphylococcus aureus) coupled to bead by cyanogen bromide activation method. |
Note | Matrix: Sepharose 4 Fast Flow, highly cross linked, 4% agarose derivative. Bead Size: 45-165 µm in size when swollen. Protein A/Sepharose: 6.0 mg Protein A per cc of drained sepharose gel. Binding Capacity: Approximately 35 mg Human IgG per cc of drained sepharose gel. Exclusion Limit (Mr): 3 x 10e7 Maximum Linear Flow Rate: < 1300 cm/hr pH Stability: Long term stability within pH range of 3 to 9. Short term stability within pH range of 2 to 10. Applications: Ideal for laboratory and process scale recovery and purification of monoclonal antibodies from dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. |
Storage | Store vial at 2-8°C prior to opening. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Reference Data |
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