Protein A (Sepharose) Staphylococcus aureus Protein

CAT#: RA100S25

Protein A (Sepharose) s. aureus protein, 25 ml

Specifications

Product Data

• Species: Staphylococcus aureus
• Concentration: lot specific
• Buffer: State: Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads).
  Buffer System: 20% (v/v) Ethanol
  Label: Sepharose (TM)
  Presentation Label: Sepharose
• Bioactivity: Specific: ~35 mg Human IgG bound per cc of drained beads.
• Preparation: Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads).
• Applications: Ideal for laboratory and process scale recovery and purification of monoclonal antibodies from dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 ul per immunoprecipitation reaction
• Protein Description: Native Protein A (Staphylococcus aureus) coupled to bead by cyanogen bromide activation method.
• Note: Matrix: Sepharose 4 Fast Flow, highly cross linked, 4% agarose derivative.
  Bead Size: 45-165 µm in size when swollen.
  Protein A/Sepharose: 6.0 mg Protein A per cc of drained sepharose gel.
  Binding Capacity: Approximately 35 mg Human IgG per cc of drained sepharose gel.
  Exclusion Limit (Mr): 3 x 10e7
  Maximum Linear Flow Rate: < 1300 cm/hr
  pH Stability: Long term stability within pH range of 3 to 9. Short term stability within pH range of 2 to 10.
  
  Applications: Ideal for laboratory and process scale recovery and purification of monoclonal antibodies from dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes.
• Storage: Store vial at 2-8°C prior to opening.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.