Protein G Streptococcus Protein
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Specifications
Product Data | |
Species | Streptococcus |
Concentration | lot specific |
Purity | This product was prepared from Chromatographically pure Protein G. |
Buffer | State: Lyophilized purified Ig fraction Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer. Label: Horseradish Peroxidase (HRP) Presentation Label: HRP |
Reconstitution | Restore with 1.0 ml of deionized water (or equivalent) |
Preparation | Lyophilized purified Ig fraction |
Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays. Recommended Dilutions: ELISA: 1/20,000-1/200,000. Western blot: 1/10,000-1/40,000. Immunohistochemistry: 1/1,000-1/5,000. Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). |
Protein Description | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A. |
Note | Caution: Do Not Add Sodium Azide. |
Storage | Store vial at 2-8°C prior to restoration. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. |
Stability | Shelf life: one year from despatch. |
Reference Data |
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Recombinant Protein Resources |
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