Tumor necrosis factor (TNF-alpha) Bovine Protein
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Specifications
Product Data | |
Species | Bovine |
Expression Host | Pichia pastoris |
Predicted MW | 17.4 kDa |
Purity | >95% pure by SDS PAGE analysis |
Buffer | Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS. Further dilutions should be made in a buffer containing carrier protein. Preservative: None Stabilizer: None |
Bioactivity | Biological: ED50 is in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant Bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D. |
Endotoxin | < 0.05 EU/µg |
Reconstitution | Restore with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. |
Preparation | Lyophilized purified protein |
Applications | ELISA: SA1000 May be used as a standard with SM2216P or SM2228B. Functional Assays. |
Protein Description | Recombinant Bovine TNF alpha expressed in Pichia pastoris. |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_776391 |
Locus ID | 280943 |
Cytogenetics | 23q22 |
Synonyms | TNF-a|TNF-alpha|TNFa |
Summary | tumor necrosis factor |
Documents
FAQs |
Resources
Recombinant Protein Resources |
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