Tumor necrosis factor (TNF-alpha) Bovine Protein

CAT#: SA1000

Tumor necrosis factor (TNF-alpha) bovine recombinant protein, 20 µg

Specifications

Product Data

• Species: Bovine
• Expression Host: Pichia pastoris
• Predicted MW: 17.4 kDa
• Purity: >95% pure by SDS PAGE analysis
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
  Buffer System: PBS. Further dilutions should be made in a buffer containing carrier protein.
  Preservative: None
  Stabilizer: None
• Bioactivity: Biological: ED₅₀ is in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant Bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.
• Endotoxin: < 0.05 EU/µg
• Reconstitution: Restore with 0.5 ml distilled water.
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Preparation: Lyophilized purified protein
• Applications: ELISA: SA1000 May be used as a standard with SM2216P or SM2228B.
  Functional Assays.
• Protein Description: Recombinant Bovine TNF alpha expressed in Pichia pastoris.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_776391
• Locus ID: 280943
• Cytogenetics: 23q22
• Synonyms: TNF-a|TNF-alpha|TNFa
• Summary: tumor necrosis factor