PTPN1 (1-321) Human Protein

CAT#: SA6014X

PTPN1 (1-321) human protein, 0.5 mg


USD 620.00

2 Weeks*

Size
    • 500 ug

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Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
Predicted MW 37.3 kDa
Concentration lot specific
Purity >95% by SDS-PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 25 mM Tris-HCl, pH 7.5, 2 mM β-mercaptoethanol, 1 mM EDTA, 1 mM DTT, 20% Glycerol
Bioactivity Specific: . Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container: The final concentrations are 10 mM Tris (pH 7.5), 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 10 mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C
3. Add recombinant PTP1B solution with various concentrations (2ug, 3ug, 4ug) in 200 ul reaction buffer.
4. Mix by inversion and Incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800 ul of 0.1N NaOH.
6. Record the absorbance at 405nm
Preparation Liquid purified protein
Protein Description The protein coding region of the catalytic domain of PTPN1 (PTP-1B) (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001265547
Locus ID 5770
UniProt ID P18031, B4DSN5
Cytogenetics 20q13.13
Synonyms PTP1B
Summary 'The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]'
Protein Families Druggable Genome, Phosphatase, Transmembrane
Protein Pathways Adherens junction, Insulin signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.