PIN1 Human Protein

CAT#: SA6039X

PIN1 human protein, 0.5 mg


USD 845.00

2 Weeks*

Size
    • 500 ug

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Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Predicted MW 18.2 kDa
Concentration lot specific
Purity >95%
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer(pH7.5) 0.1M NaCl, 5mM DTT, 20%Glycerol.
Bioactivity Biological: Specific activity is >330nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
Endotoxin < 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation Liquid purified protein
Applications SDS-PAGE
Note Activity Assay
1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
2. Add 10ul of recombinant PIN 1 protein with 1ug in assay buffer.
3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_006212
Locus ID 5300
UniProt ID Q13526
Cytogenetics 19p13.2
Synonyms DOD; UBL5
Summary 'Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]'
Protein Families Druggable Genome
Protein Pathways RIG-I-like receptor signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.