HAGH (NM_005326) Human Recombinant Protein

CAT#: TP301109

Recombinant protein of human hydroxyacylglutathione hydrolase (HAGH), transcript variant 1

Product Images

Coomassie blue staining of purified HAGH protein (Cat# TP301109). The protein was produced from HEK293T cells transfected with HAGH cDNA clone (Cat# RC201109) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC201109 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVVGRGLLGRRSLAALGAACARRGLGPALLGVFCHTDLRKNLTVDEGTMKVEVLPALTDNYMYLVIDDET
  KEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHL
  STLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGR
  LPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTV
  QQHAGETDPVTTMRAVRREKDQFKMPRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 30.5 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_005317
• Locus ID: 3029
• UniProt ID: Q16775
• Cytogenetics: 16p13.3
• Refseq Size: 1552
• Refseq ORF: 924
• Synonyms: GLO2; GLX2; GLXII; HAGH1
• Summary: The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
• Protein Families: Druggable Genome
• Protein Pathways: Pyruvate metabolism