RDH11 (NM_016026) Human Recombinant Protein

CAT#: TP305003

Recombinant protein of human retinol dehydrogenase 11 (all-trans/9-cis/11-cis) (RDH11)

Product Images

The expression levels of microsomal RDH11 in four human livers are shown using either recombinant protein standard (OriGene TP305003) or the AQUA method as calibrators in LC-MS/MS assays. Figure cited from Anal Chem, PMID: 26606514

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  Coomassie blue staining of purified RDH11 protein (Cat# TP305003). The protein was produced from HEK293T cells transfected with RDH11 cDNA clone (Cat# RC205003) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC205003 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVELMFPLLLLLLPFLLYMAAPQIRKMLSSGVCTSTVQLPGKVVVVTGANTGIGKETAKELAQRGARVYL
  ACRDVEKGELVAKEIQTTTGNQQVLVRKLDLSDTKSIRAFAKGFLAEEKHLHVLINNAGVMMCPYSKTAD
  GFEMHIGVNHLGHFLLTHLLLEKLKESAPSRIVNVSSLAHHLGRIHFHNLQGEKFYNAGLAYCHSKLANI
  LFTQELARRLKGSGVTTYSVHPGTVQSELVRHSSFMRWMWWLFSFFIKTPQQGAQTSLHCALTEGLEILS
  GNHFSDCHVAWVSAQARNETIARRLWDVSCDLLGLSID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 35.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: MS digestion standard (PMID: 26606514)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_057110
• Locus ID: 51109
• UniProt ID: Q8TC12, A0A0S2Z583
• Cytogenetics: 14q24.1
• Refseq Size: 2587
• Refseq ORF: 954
• Synonyms: ARSDR1; CGI82; HCBP12; MDT1; PSDR1; RALR1; RDJCSS; SCALD; SDR7C1
• Summary: The protein encoded by this gene is an NADPH-dependent retinal reductase and a short-chain dehydrogenase/reductase. The encoded protein has no steroid dehydrogenase activity. [provided by RefSeq, Nov 2011]
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism