LRRC50 (DNAAF1) (NM_178452) Human Recombinant Protein

CAT#: TP305452

Recombinant protein of human leucine rich repeat containing 50 (LRRC50)

Product Images

Coomassie blue staining of purified DNAAF1 protein (Cat# TP305452). The protein was produced from HEK293T cells transfected with DNAAF1 cDNA clone (Cat# RC205452) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC205452 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPEPSEPATGGAAELDCAQEPGVEESAGDHGSAGRGGCKEEINDPKEICVGSSDTSYHSQQKQSGDNGS
  GGHFAHPREDREDRGPRMTKSSLQKLCKQHKLYITPALNDTLYLHFKGFDRIENLEEYTGLRCLWLQSNG
  IQKIENLEAQTELRCLFLQMNLLRKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNHLETV
  EDIQHLQECLRLCVLDLSHNKLSDPEILSILESMPDLRVLNLMGNPVIRQIPNYRRTVTVRLKHLTYLDD
  RPVFPKDRACAEAWARGGYAAEKEERQQWESRERKKITDSIEALAMIKQRAEERKRQRESQERGEMTSSD
  DGENVPASAEGKEEPPGDRETRQKMELFVKESFEAKDELCPERPSGEEPPVEAKREDGGPEPEGTLPAET
  LLLSSPVEVKGEDGDGEPEGTLPAEAPPPPPPVEVKGEDGDQEPEGTLPAETLLLSPPVKVKGEDGDREP
  EGTLPAEAPPPLPLGAAREEPTPQAVATEGVFVTELDGTRTEDLETIRLETKETCCIDDLPDLEDDDETG
  KSLEDQNMCFPKIEVISSLSDDSDPELDYTSLPVLENLPTDTLSNIFAVSKDTSKAARVPFTDIFKKEAK
  RDSEIRKQDTKSPRPLIQELSDEDPSGQPLMPPTCQRDAAPLTSTGDRDSDFLAASSPVPTESAATPPET
  CVGVAQPSQALPTWDLTAFPAPKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 79.8 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_848547
• Locus ID: 123872
• UniProt ID: Q8NEP3, A0A140VJN4
• Cytogenetics: 16q24.1
• Refseq Size: 2451
• Refseq ORF: 2175
• Synonyms: CILD13; DAU1; LRRC50; ODA7; swt
• Summary: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]